IgH mutation frequency in uninfected B cell subsets and derived LCLs.

<p>Histograms show the number of mutations among IgH clonotypes amplified from naïve, non-switched memory and switched memory B cell preparations before EBV infection (open bars) and among sequences amplified from the derived limiting dilution LCLs (shaded bars). Mutations were only scored between IgH codons 9–92 (i.e. excluding CDR3). Where LD LCL cultures yielded multiple sequences with differing numbers of mutation within the same CDR3 clone, the sequence with the maximum number of mutations was scored.