Additional file 2: of Improving saliva shotgun metagenomics by chemical host DNA depletion

Figure S2. Optimization of lyPMA conditions for human DNA depletion. qPCR analysis of the relative abundance of the human-specific PTGER2 gene normalized to raw saliva across methods of selective mammalian cell lysis (A) and PMA concentration (B). qPCR analysis of the fold change of the bacteria-specific 16S rRNA gene normalized to raw saliva across methods of selective mammalian cell lysis (C) and PMA concentration (D). SS = slow centrifugation (30 s at 2500 g), son = sonication (15 min at 60 Hz), H2O = osmotic lysis with pure water. (PNG 274 kb