Additional file 6: of Improving saliva shotgun metagenomics by chemical host DNA depletion
- Clarisse Marotz (4890046)
- Jon Sanders (3244497)
- Cristal Zuniga (4890043)
- Livia Zaramela (4741440)
- Rob Knight (11635)
- Karsten Zengler (247723)
Publication date
February 2018
DOIAbstract
Figure S6. Host depletion via PMA treatment is possible for cryopreserved samples. Raw saliva samples were aliquoted and either frozen immediately at − 20 °C or mixed with a final concentration of 20% glycerol for cryopreservation. The percentage of human reads was assessed by Bowtie2, and the top 15 most abundant genera were assessed by MetaPhlAn2. (PNG 550 kb
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