Expression of CHRN subunits, taste receptors, and intracellular signaling intermediates in HBO and HEK293 cells.

<p>Consensus primers to amplify CHRN subunits, GPCRs, and downstream signaling intermediate were designed based on the published sequences and are shown in Tables <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0194089#pone.0194089.t001" target="_blank">1</a> and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0194089#pone.0194089.t002" target="_blank">2</a>. <b>(A)</b> Based on the predicted sizes of the PCR products (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0194089#pone.0194089.t001" target="_blank">Table 1</a>), mRNAs for the CHRNA3, CHRNA4, CHRNA5, CHRNA6, CHRNA7, CHRNB2, and CHRNB4 were detected in HBO cells. <b>(B)</b> In addition, mRNAs for T1R1, T1R3, T2R38, PLCβ2, and TRPM5 were detected in HBO cells. <b>(C)</b> In Western blot analysis using specific AChRα4 and AChRα5 antibodies, the expression of α4 and α5 proteins were detected in HBO and HEK293 cell lysates. <b>(D)</b> Based on the predicted sizes of the PCR products (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0194089#pone.0194089.t001" target="_blank">Table 1</a>), mRNAs for the CHRNA3, CHRNA4, CHRNA5, CHRNA6, CHRNB2 and CHRNB4 were detected in HEK293 cells. In addition, mRNAs for T2R38 and TRPM5 were detected.