Genome locations of the essential ncRNAs and the expression of nearby genes.

<p>The ncRNA deletions are indicated by vertical black dotted lines and the direction of KanMX deletion cassette expression is indicated with a grey arrow. Red arrows are protein coding genes and the arrows encompass the annotated 5’ and 3’ UTR regions. The essentiality of nearby protein coding genes is indicated. (<b>A</b>) SUT527 full deletion. Relative expression of <i>SEC4</i> in the wild type diploid background is represented by grey bar and <i>SEC4</i> expression in the SUT527 diploid deletion background is represented by black bar. <i>SEC4</i>: <i>p</i> = 0.02. (<b>B</b>) Overlapping SUT259/691 deletion. Relative expression of <i>EMP46</i> and <i>GAL2</i> in the wild type diploid background is represented by grey bars and <i>EMP46</i> and <i>GAL2</i> expression in the SUT259/691 diploid deletion background is represented by black bars. <i>EMP46</i>: p = 0.02, <i>GAL2</i>: p = 0.02. (<b>C</b>) SUT075 partial deletion. Relative expression of <i>PRP3</i> in the wild type diploid background is represented by grey bar and <i>PRP3</i> expression in the SUT075 diploid deletion background is represented by black bar. <i>PRP3</i>: <i>p</i> = 0.04. (<b>D</b>) SUT367 full deletion. Relative expression of <i>RPL3</i> in the wild type diploid background is represented by grey bar and <i>RPL3</i> expression in the SUT367 diploid deletion background is represented by black bar. <i>RPL3</i>: <i>p</i> = 0.02. The fold change (2^) in expression, relative to the wild-type was calculated using the ΔΔCт method and <i>ACT1</i> as a reference gene. Error bars were calculated using three independent biological samples. P values were calculated using the Welch two sample t-test.