Détatouage au laser: le remord dans la peau []

Les lasers Q-switched sont actuellement le traitement de choix pour l’ablation des tatouages. Choisir le bon laser en fonction de la couleur du tatouage du patient et de son type de peau est primordial pour obtenir de bons résultats. Dans cette revue, nous détaillerons les différents types de lasers utilisés dans le détatouage, de même que leurs indications. Cet article passera également en revue les différentes complications possibles, telles que les dyspigmentations, les réactions allergiques, l’assombrissement de certaines encres, etc., ainsi que les moyens de les prévenir. [ Q-switched lasers represent the best treatment option for tattoo removal. The selection of the adequate laser, depending upon the tattoo color and skin phototype, is essential to achieve optimal results. In this article, the different lasers used for tattoo removal as well as the potential side effects and their prevention will be discussed.]]]> Laser Therapy/adverse effects; Laser Therapy/methods; Lasers/adverse effects; Patient Satisfaction; Tattooing/adverse effects; Tattooing/classification; Treatment Outcome; Pikosekundenlaser; Q-Switch-Laser; Q-switched laser; Tatouage; Tattoo; Tattoo-Laserentfernung; détatouage; laser Q-switched; laser picosecond; picosecond laser; tattoo removal fre oai:serval.unil.ch:BIB_26C440FD1F1B 2023-07-29T01:19:56Z <oai_dc:dc xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:xs="http://www.w3.org/2001/XMLSchema" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd"> https://serval.unil.ch/notice/serval:BIB_26C440FD1F1B Isolation and Culture of Human Adipose-Derived Stem Cells With an Innovative Xenogeneic-Free Method for Human Therapy. info:doi:10.3791/65104 info:eu-repo/semantics/altIdentifier/doi/10.3791/65104 info:eu-repo/semantics/altIdentifier/pmid/36806184 Guiotto, M. Palombella, S. Brambilla, S. Applegate, L.A. Riehle, M. Hart, A. Raffoul, W. di Summa, P.G. info:eu-repo/semantics/article article 2023-02-03 Journal of visualized experiments, no. 192 info:eu-repo/semantics/altIdentifier/eissn/1940-087X urn:issn:1940-087X <![CDATA[Considering the increasing impact of stem cell therapy, biosafety concerns have been raised regarding potential contamination or infection transmission due to the introduction of animal-derived products during in vitro manipulation. The xenogeneic components, such as collagenase or fetal bovine serum, commonly used during the cell isolation and expansion steps could be associated with the potential risks of immune reactivity or viral, bacterial, and prion infection in the receiving patients. Following good manufacturing practice guidelines, chemical tissue dissociation should be avoided, while fetal bovine serum (FBS) can be substituted with xenogeneic-free supplements. Moreover, to ensure the safety of cell products, the definition of more reliable and reproducible methods is important. We have developed an innovative, completely xenogeneic-free method for the isolation and in vitro expansion of human adipose-derived stem cells without altering their properties compared to collagenase FBS-cultured standard protocols. Here, human adipose-derived stem cells (hASCs) were isolated from abdominal adipose tissue. The sample was mechanically minced with scissors/a scalpel, micro-dissected and mechanically dispersed in a 10 cm Petri dish, and prepared with scalpel incisions to facilitate the attachment of the tissue fragments and the migration of hASCs. Following the washing steps, hASCs were selected due to their plastic adherence without enzymatic digestion. The isolated hASCs were cultured with medium supplemented with 5% heparin-free human platelet lysate and detached with an animal-free trypsin substitute. Following good manufacturing practice (GMP) directions on the production of cell products intended for human therapy, no antibiotics were used in any culture media