Far-red fluorescent reporters are functional in <i>M. marinum</i> strain M.

<p>Fluorescent reporters were expressed from P_smyc in <i>M. marinum</i> and assayed in liquid culture grown either in the dark or in the light. Cultures were diluted (from left to right) to an OD₅₈₀ of 0.25, 0.10, 0.05 and 0.01, representing cell densities of 9.8×10⁵, 4.2×10⁵, 1.9×10⁵ and 6.5×10⁴ CFU/ml respectively. Fluorescence was quantified at the following wavelengths: mCherry 587/610 nm, Turbo-635 588/635 nm. Data are the mean and standard deviation from three independent transformants. A value of 1015 corresponds to saturation of the machine. The reporter and promoter backbone are indicated.