Detection of fluorescent reporters in <i>E. coli</i> DH5α.

<p><i>E. coli</i> transformants were cultured and diluted (from left to right) to an OD₅₈₀ of 0.25, 0.10, 0.05 and 0.01, representing cell densities of 5.5×10⁶, 1.5×10⁶, 7.7×10⁵ and 1.1.×10⁵ per mL respectively. Fluorescence was quantified at the following wavelengths: mCherry 587/610 nm, mKat 588/635 nm, mPlum 590/649 nm, tdTomato 554/581 nm, Turbo-635 588/635 nm. Data are the averages and standard deviations from three independent transformants. A value of 1015 corresponds to saturation of the machine. The reporter, promoter and vector backbone are indicated. WT = wild-type oriM; ΔHC – high copy number derivative oriM.