MicroRNAs can affect particle to infectivity ratio of CVA21.

<p>(A) Fold change in CVA21 142T RNA as determined by qRT-PCR of viral RNA from supernatant of infected cells at indicated time pre/post miRNA mimic addition. Fold decrease expressed as ratio of CVA 142T RNA in the presence of miR-142 mimic/CVA 142T RNA in the presence of control miRNA mimic (at indicated MOI). (B) Protein assay of sucrose-purified virus collected in the presence of miRNA mimics added at 4 hours prior, or 0, 3, or 6 hours after virus infection at MOI = 1. (C) Western blot with anti-CVA21 of sucrose-purified supernatant run on 12.5% SDS-PAGE gel from miRNA mimic timecourse at MOI = 1. Lane 1-Control mimic -4 hrs pi + CVA 142T; Lane 2- 142 mimic -4 hrs pi + CVA 142T; Lane 3- Control mimic 0 hrs pi + CVA 142T; Lane 4- 142 mimic 0 hrs pi +CVA 142T; Lane 5- Control mimic +3 hrs pi + CVA 142T; Lane 6- 142 mimic +3 hrs pi + CVA 142T; Lane 7- Control mimic +6 hrs pi + CVA 142T; Lane 8- 142 mimic +6 hrs pi +CVA 142T; Lane 9- Mock infected. BLD- below limit of detection. #p = ns (vs. H1-HeLa + Control mimic). pi: post infection.