Effects of protein kinase inhibitors on V-ATPase-mediated surfactant secretion.

<p>Overnight cultured type II cells were treated with 20 nM Bafilomycin A1 (Baf A1) in the presence or absence of staurosporine (Stauro, 10 nM) (Panel A), KN-62 (10 µM) (Panel B) or 20 µM terbutaline (Panel C). PC secretion was assayed. Data shown are means ± SE. *P<0.05 <i>v.s.</i> Control; #P<0.05 <i>v.s</i> Baf A1 (ANOVA/Newman-Keuls Multiple Comparison Tests, n = 3 independent cell preparations for Panel A and n = 4 independent cell preparations for Panel B). *<i>P</i><0.05 <i>v.s.</i> Control (basal); ^#P<0.05 <i>v.s.</i> Baf A1 (basal); ^$P<0.05 <i>v.s.</i> Control (Terbutaline) (ANOVA/Newman-Keuls Multiple Comparison Tests, n = 3–4 independent cell preparations for Panel C).