<i>Per1-luc</i> Expression in Peripheral Tissues Explanted from C57BL/6J <i>Per2^−/−</i> and <i>Per3^−/−</i> Mice.

<p>Representative baseline-subtracted bioluminescence rhythms in pituitary (A) and lung (B) explants from <i>Per2^+/+</i> (black trace; top panel; n = 6) and <i>Per2^−/−</i> (red trace; top panel; n = 4-6) mice and from <i>Per3^+/+</i> (black trace; bottom panel; n = 4) and <i>Per3^−/−</i> (red trace; bottom panel; n = 5) mice. The lighting condition of the previous light/dark cycle is indicated for the first day; open bars are light and black bars are dark. The period of pituitary (C) and lung (D) explants was determined by fitting a regression line to the acrophase of the <i>Per1</i>-<i>luc</i> rhythm. The phase of pituitary (E) and lung (F) explants was designated as the first peak of <i>Per1-luc</i> expression <i>in vitro</i> and is plotted relative to the light-dark cycle before culture, where 0 h is the time of lights on and 12 h is the time of lights off (open bars are light and black bars are dark). All data are presented as the mean±SD; *<i>p</i><0.05, ***<i>p</i><0.001.