<i>Bmal1</i>-driven bioluminescence rhythms of transgenic mouse tissues and fibroblasts.

<p>(A) Representative bioluminescence recordings of SCN, lung, adrenal gland, and thyroid gland tissues from <i>Bmal1^ELuc-A1</i> mice. The <i>x</i> axis indicates the time after the last lights on. (B) Data shown in (A) were detrended to reduce noise and baseline trends, as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0037093#s4" target="_blank">Materials and Methods</a>. Clear circadian rhythms were observed in each tissue. (C) Average peak times (means ± SD) were plotted against the time after the last lights on. Peaks were determined from the detrended data. (D) Average periods (means ± SD). The average phase and period were determined for SCN (n = 6), lung (n = 10), adrenal gland (n = 10), and thyroid gland (n = 6) tissues. One or two explants were obtained from each animal. (E–F) Representative bioluminescence recordings of Bmal1-RED fibroblasts after dexamethasone treatment (E) and without dexamethasone treatment (F). Open bar, the previous light period. Filled bar, the previous dark period. cpm, counts per minute. Bars indicate SD. *: <i>P</i><0.05, **: <i>P</i><0.01, Kruskal-Wallis <i>H</i>-test followed by Mann-Whitney <i>U</i>-test with Bonferroni correction. Data were compared with SCN. Adrenal, adrenal gland. Thyroid, thyroid gland.