Effects of <i>smyhc1</i> knockdown or <i>hsp90α1</i> mutation on myosin thick filament organization in skeletal muscles of zebrafish embryos.

<p>A–C. Anti-MyHC antibody (F59) staining shows the organization of thick filaments in trunk slow muscles of control (A), <i>smyhc1</i> knockdown (B), or <i>slo^tu44c</i> mutant (C) embryos at 48 hpf. D–F. Anti-MLC antibody (F310) staining shows the organization of thick filaments in trunk fast muscles of control (D), <i>smyhc1</i> knockdown (E), or <i>slo^tu44c</i> mutant (F) embryos at 72 hpf. Note, fast fibers project with a 30 degree angle with respect to the axial structure, whereas slow fibers project in parallel to the axial structure. G, H. Anti-MyHC antibody (F59) staining shows the rescue of thick filaments in smyhc1 knockdown zebrafish embryos co-injected with <i>ef1a:smyhc1</i> DNA construct (G), or ATG-MO alone (H). Scale bar = 25 µm in A, 10 µm in G.