The effect of <i>smyd1a</i> knockdown on the sarcomere organization in fast muscles.

<p>Zebrafish embryos co-injected with <i>smyd1a</i> E8I8 and E9I9 MOs were fixed at 28, 48 and 72 hpf. The sarcomere organization of thin filaments and Z-lines was analyzed by immunostaining with anti-α-actin and anti-α-actinin antibody, respectively, and followed by FTIC-labeled secondary antibody. The images represent side view of trunk muscles around segment 10. <b>A–C.</b> Lateral view of thin filament organization in skeletal muscle fibers of control-MO injected embryos at 28 (A), 48 (B) and 72 (C) hpf. <b>D–F.</b> Lateral view of thin filament organization in skeletal muscle fibers of <i>smyd1a</i> MO injected embryos at 28 (D), 48 (E) and 72 (F) hpf. <b>G–I.</b> Lateral view of Z-line organization in skeletal muscle fibers of control-MO injected embryos at 28 (G), 48 (H) and 72 (I) hpf. <b>J–L.</b> Lateral view of the Z-line organization in skeletal muscle fibers of <i>smyd1a</i> MO injected embryos at 28 (J), 48 (K) and 72 (L) hpf. Scale bars: 20 µm in D–F and J–L.