RWE-induced upregulation of GTPases, Socs1 and Gadd45g.

<p>(<b>A</b>) Sensitized Balb/c mice were challenged with either PBS (◊) or RWE (▪). Lungs were harvested from mice sacrificed at different time points. Total RNA was extracted from the lungs and subjected to real-time quantitative PCR analyses using gene-specific primers. Transcript abundance is presented here as a function of time after normalizing to 10⁶ β-actin transcripts. Data are represented as mean±SEM; * = P<0.05. (<b>B</b>) Schematic representation of the time frame for which the Th2 genes (upper group) and Th1 genes (lower group) remain upregulated after RWE challenge.