rBPI₂₁-induced membrane leakage.

<p>Carboxyfluorescein fluorescence increase measured 20 min after rBPI₂₁ addition in POPC (black circles), POPC∶LPS 80∶20 (red circles), POPC∶POPG 80∶20 (green circles), POPC∶POPG 55∶45 (orange circles), POPC∶POPG∶LPS 60∶20∶20 (brown circles) and POPG (blue circles) liposomes. Bars represent the range from at least two independent experiments. The differences in leakage percentages for rBPI₂₁ 9 µM were statistically significant for the following data pairs: POPC/POPC∶LPS (80∶20), POPC/POPC∶POPG (80∶20), POPC/POPC∶POPG (55∶45), POPC/POPG, POPC/POPC∶POPG∶LPS (60∶20∶20), POPC∶LPS (80∶20)/POPG∶POPG (55∶45), POPC∶LPS (80∶20)/POPG, POPC∶POPG (80∶20)/POPC∶POPG (55∶45), POPC∶POPG (80∶20)/POPG, POPC∶POPG (55∶45)/POPC∶POPG∶LPS (60∶20∶20) and POPC∶POPG∶LPS (60∶20∶20)/POPG.