Dye release from LUVs comprised of 12 mol % BMP at pH 4.5, varying β₂m: lipid concentration ratio and cholesterol content.

<p>(<i>A</i>) Dye release measured for LUVs (5 µM lipid) at different monomer equivalent concentrations of β₂m. (<i>B</i>) Dye release measured for varying lipid equivalent concentrations of LUVs at 6 µM monomer equivalent concentration of β₂m. (<i>C</i>) Dye release from LUVs (5 µM lipid) comprising 36 POPC: 20 POPE: 7 SM (mol/mol), 12 mol % BMP plus varying concentrations of cholesterol at 6 µM monomer equivalent concentration of β₂m. Dye release was measured 10 min after addition β₂m monomer (<i>circle, blue</i>), fragmented fibrils (<i>square, red</i>) and unfragmented fibrils (<i>triangle, green</i>) in <i>Assay Buffer</i> pH 4.5, 37°C. Error bars represent 1 S.D. from three replicates. Open symbols corresponds to dye release measured for 6 µM monomer equivalent β₂m samples with 5 µM lipid equivalent concentration of LUVs containing 25 mol % cholesterol, as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0104492#pone-0104492-g003" target="_blank">Fig. 3</a>.