Effect of MyD88 expression on KC, TNF-α and IL-6 synthesis by alveolar macrophages challenged with <i>P. aeruginosa</i>.

<p>Alveolar macrophages (AM) collected from WT and MyD88^–/– mice were infected for 4 h with increasing concentrations (number of bacteria/well) of <i>P. aeruginosa</i> wild-type strain PAK. <i>NS</i>, non-stimulated cells cultured with medium only. LPS (1 µg/mL) and flagellin (20 ng/mL) were used as control agonists to stimulate the cells. Values represent means±SEM of three to five experiments performed in triplicate. *, <i>p</i><0.05 when compared with the corresponding WT values.