MβCD treatment does not prevent correct regional targeting of the axons innervating an identified glomerulus (glomerulus X).

<p>Untreated (A–C) and MβCD-treated (D–F) animals. A,B: Stage 7. An antibody to human ankyrin B (yellow) labels ORN axons targeting a single glomerulus located dorso-posteriorly close to primary neurites of the medial cluster of AL neurons (outlined with dashed line). Neuronal cell bodies and glial nuclei labeled with Syto 13 (blue). C: Double labeling with the ankyrin B antibody (magenta) and the MFas II antibody (green) demonstrates that glomerulus X (arrow) is Fas II-negative. D–F: 7.5 mg MβCD, injected at early stage 3, brain dissected at stage 7. Ank B axons target a single glomerulus located near the primary neurites of the medial cluster of AL neurons although the shape of the glomerulus is variable and the pattern of fasciculation in the SZ is somewhat abnormal.