Analysis of sequences of enriched shRNA.

<p>(A) Pie chart representing the frequency of shRNA occurrence among the 140 clones sequenced. 2-3X refers to the pool of sequences represented two or three times. Other refers to unique sequences. (B) Sequences of the shRNAs present at the highest frequency. CACC represents the 3′ end of the human U6 promoter. TTCAAGAGA represents the loop sequence. TTTTT represents the termination sequence. Number in parentheses represents the frequency of occurrence out of 140 total sequences. The underlined sequence represents a mismatch within the inverted repeat.