Analysis of thymocyte development in Hes1 transgenic mice.

<p>(A) Cell numbers of the different thymocyte populations are shown as an average of 4 mice at 3–5 weeks of age. (B) FACS analysis was performed on thymocytes isolated from individual wild type (WT) or Hes1 transgenic littermates and stained with indicated antibodies. (C) CD4⁺CD8⁺ DP cells shown in (B) were further analyzed for CD25 expression. (D) Thymocytes isolated from WT or Hes1 transgenic littermates were stained with antibodies against CD8, B220, TCRγ/δ, GR1 and CD3. The lineage negative thymocytes were further stained with anti-CD44 and anti-CD25. Percentages of cells in each gate are as labeled.