CyP facilitate exposure of the RG-1 epitope after cell attachment.

<p>HPV16 pseudovirus was bound to HaCaT cells for 2 h at 4°C and chased for 4 h at 37°C in presence or absence of NIM811. (A) Cells were subsequently stained with L2-specific RG-1 and L1-specific K75 antibody. All images were taken using the same settings. (B) RG-1– and K75–specific signal strength of randomly selected cells (n>15 for each group) was measured. RG-1 reactivity normalized to K75 signal strength is plotted after subtraction of background signal. The graph shows quantifications from one experiment. However, the experiment was repeated three times with similar outcomes.