Volcano Plots of inflammatory gene array changes.

<p>For each plot, fold change in expression for each of the inflammatory cytokines studied (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0005835#pone-0005835-t002" target="_blank">Table 2</a>) is plotted on the abscissa while t-test significance is plotted on the y-axis. In all three plots, comparison of gene expression is made to control group (Sal (s.c.)/Sal(intraluminal). A significant change was arbitrarily defined as a 10-fold difference (vertical lines) <u>AND</u> a p<0.001 (horizontal line). Using these criteria then, significant changes have been plotted using red markers, while non-significant changes are plotted using blue markers. A) Substance P treatment with saline as an intraluminal treatment (SP = Substance P(s.c.)/Saline (intraluminal) upregulated many inflammatory genes (red points in upper right quadrant). B) Intraluminal CD74 ab treatment, in SP treated rats (SP+CD74) reduced the number of inflammatory genes upregulated by SP. C) Similarly, intraluminal GRP78 ab treatment also reduced the number of genes upregulated by SP with some showing significant downregulation (red points in upper left quadrant).