Volcano plot and the number and biological processes associated with differentially expressed genes in the colon tissue of high-fat diet fed C57BL/6J mice.

<p>The volcano plot represents the relationship between the fold change and significance between the normal diet and high-fat diet groups; the x-axis indicates the difference in gene expression between two dietary groups as log₂ of their fold change, and the y-axis indicates the negative log₁₀ of P-values for the <i>t</i>-test (<b>A</b>). Twenty-five genes with a <i>t</i>-test P-value < 0.05 and a log₂ fold change of ≥ 0.5849 (fold change ≥ 1.5) or ≤ -0.5849 (fold change ≤ 0.66) were selected and further analyzed using the Database for Annotation, Visualization, and Integrated Discovery (DAVID) for functional annotation analysis (<b>B</b>, <b>C</b>).