Relationship between the UPS and inclusion formation.

<p>(A) Immunofluorescent double-staining of R6/2; GFPu brain sections shows that the presence of nuclear inclusion bodies does not correlate with the intensity of GFPu immunofluorescence in CA1 region of the hippocampus, the piriform cortex or the cortex (B). Quantification of GFPu immunofluorescence in nuclei with or without an inclusion body confirms that there is no relationship between inclusion body formation and impairment of the UPS in R6/2 mice. Sections were stained with the nuclear fluorescent dye TOPRO-3. Error bars represent the standard errors of the mean. Scale bars are 6 µM.