GFPu does not accumulate in the R6/2 brain.

<p>(A) Schematic showing the GFPu construct under control of the mouse prion promoter (PrP). GFPu protein is composed of GFP appended with a 16 amino acid C-terminal degradation signal, the CL-1 degron. (B) Western blot analysis and densitomeric quantification reveals no increase in steady-state levels of GFPu in 12 week R6/2 brains. α-tubulin was used as a loading control. (C) Expression of the GFPu transgene is unchanged in the 12-week-old R6/2 brain. Error bars represent the standard error of the mean.