Liver cCD8α⁺ DC are more efficient than splenic cCD8α⁺ DC in inducing differentiation of and IFN-γ production by CD8⁺ T cells.

<p>Differentiation and induction of CD8⁺ T cell function is MHC class I- and IL-12 dependent. HMNC or splenic MNC were prepared after the 3° immunization as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0005075#pone-0005075-g003" target="_blank">Fig. 3</a>. CD8α⁺ DC were purified as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0005075#pone-0005075-g001" target="_blank">Figs 1</a> and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0005075#pone-0005075-g003" target="_blank">3</a>. Splenic (A and C) or hepatic (B and D) cCD8α⁺ DC were co-cultured for 4 days either alone or with purified CD8⁺ T cells from the livers (open bars) or spleen (filled bars) of naïve mice. Cells were harvested, stained with the appropriate mAb and analyzed by flow cytometry. Culture supernatants were analyzed for IFNγ by ELISA. (A and B) Results show the mean % of CD8⁺ T_EM in the gated CD3⁺CD8⁺ T cell population and (C and D) the amount of IFNγ in the culture supernatant. Data are representative of two individual experiments. (E and F) Liver cCD8α⁺ DC were co-cultured with naïve splenic CD8⁺ T cells in the presence or absence of anti-IL-12 (clone C17.1) and/or anti-MHC class I (clone 28-8-6) mAbs for 4 days. (E) Results show the mean % of CD8⁺ T_EM in the gated CD3⁺CD8⁺ T cell population and (F).the amount of IFNγ. Data is representative of two individual experiments.