Gef1/tubulin immunofluorescence in cells without deciliation.

<p>Top panel: K5KOAs.40 cells with 0 µg/ml Cd²⁺; middle panel: K5KOAs.40 cells with 0.5 µg/ml Cd²⁺ (12 h); bottom panel: Inv2 cells with 0.5 µg/ml Cd²⁺ (12 h). Right panels: Enlarged cilia showing the presence or absence of punctate pattern of Gef1 antibody (red) localization offset from the continuous tubulin localization (green). Scale bars: (left) 10 µm; (right) 1 µm. In KO cells treated with Cd²⁺, unlike Kin5, Gef1 remains in the cilia.