Bradykinin stimulation of PS-1 (M146L) AD fibroblasts results in decreased JNK activation relative to control fibroblasts.

<p>PS-1 (M146L) AD and control human skin fibroblasts treated with 25 nM BK (A) or 250 nM BK (B) for 0–30 min were immunoblotted for active JNK with phospho-epitope specific antibodies and analyzed by ECL. Fold activation was quantified as a percentage of basal activation in buffer treated cells (0 min) normalized to tubulin and is graphically represented for active JNK (A–B). Representative immunoblots shown display differential JNK activation profiles between PS-1 (M146L) AD and control fibroblasts (C). All graphs show mean±S.E. Statistical analysis was performed via t-test with *p-value<0.05 and ***p<0.0005; n = 4.