Muscle total IRS-1 protein (A) and IRS-1 pS307 inhibitory phosphorylation (B) in GHRKO (KO) and normal (N) mice fed ad libitum (AL) or 30% calorie restriction (CR) for one year was determined using ELISA.

Effects of Long-Term Caloric Restriction on Early Steps of the Insulin-Signaling System in Mouse Skeletal Muscle

Danila P. Argentino
O P. Dominici
Khalid Al-regaiey
Michael S. Bonkowski
Andrzej Bartke
Daniel Turyn

December 2014

In this study, we analyzed the effects of long-term (14 months) caloric restriction (CR) on the firs...

Skeletal muscle enzyme activity.

Christian Roy (303775)
Sabina Paglialunga (382945)
Gert Schaart (194711)
Esther Moonen-Kornips (382946)
Ruth C. Meex (382947)
Esther Phielix (284460)
Joris Hoeks (194696)
Matthijs K. C. Hesselink (357549)
Katherine Cianflone (155973)
Patrick Schrauwen (42320)

February 2013

HADH (A), CS (B) activities and HADH/CS ratio (C) for WT (white bars) and C5L2KO (black bars). Da...

Liver expression of LDLR and ABCA1 protein in mice with hepatic MTP knockdown.

Stephanie M. Marshall (223125)
Kathryn L. Kelley (505769)
Matthew A. Davis (505770)
Martha D. Wilson (350726)
Allison L. McDaniel (223120)
Richard G. Lee (505771)
Rosanne M. Crooke (361540)
Mark J. Graham (361539)
Lawrence L. Rudel (223108)
J. Mark Brown (505772)
Ryan E. Temel (223114)

January 2014

Following 6 weeks of control ASO or MTP ASO treatment, liver was collected for immunoblot analysi...

Muscle insulin signaling cascade total proteins and phospho-proteins in response to insulin stimulation (10 IU/kg) versus saline treated controls in GHRKO (KO) and normal (N) mice fed ad libitum (AL) or 30% calorie restriction (CR) for one year.

Michael S. Bonkowski (374374)
Fernando P. Dominici (374376)
Oge Arum (374378)
Juliana S. Rocha (374380)
Khalid A. Al Regaiey (374381)
Reyhan Westbrook (374382)
Adam Spong (374384)
Jacob Panici (374386)
Michal M. Masternak (68106)
John J. Kopchick (331606)
Andrzej Bartke (68107)

February 2013

Levels of total insulin receptor (IR; A), IR pY1158 (B), AKT1 (D), and AKT1 pS473 (E) were determ...

Metabolic parameters of male GHRKO (KO) and normal (N) mice fed ad libitum (AL) or subjected to 30% calorie restriction (CR) for one year.

Michael S. Bonkowski (374374)
Fernando P. Dominici (374376)
Oge Arum (374378)
Juliana S. Rocha (374380)
Khalid A. Al Regaiey (374381)
Reyhan Westbrook (374382)
Adam Spong (374384)
Jacob Panici (374386)
Michal M. Masternak (68106)
John J. Kopchick (331606)
Andrzej Bartke (68107)

February 2013

Panel (A) shows time-course changes in body weight over a one year period. After one year of CR, ...

Increased inflammation in skeletal muscle of KI mice is associated with reduced basal IRS1-PKB signalling.

Paul J. Meakin (800118)
Vicky L. Morrison (800119)
Claire C. Sneddon (800120)
Terhi Savinko (800121)
Liisa Uotila (800122)
Susan M. Jalicy (800123)
Jennie L. Gabriel (800124)
Li Kang (494757)
Michael L. J. Ashford (800125)
Susanna C. Fagerholm (800126)

September 2015

(A-C) Representative immunoblots for p-IRS1, IRS1, p-PKB and PKB, with quantification of immunobl...

Effect of procyanidins, EC and C3G on phosphorylation of IRS-1 in skeletal muscle of mice.

Yoko Yamashita (1948978)
Liuqing Wang (3106479)
Fumio Nanba (1948987)
Chiaki Ito (1948984)
Toshiya Toda (3106476)
Hitoshi Ashida (1948975)

September 2016

ICR mice were treated as described in <a href="http://www.plosone.org/article/info:doi/10.1371/jo...

Liver PI3K subunit abundance in GHRKO (KO) and normal (N) mice fed ad libitum (AL) or 30% calorie restriction (CR) for one year.

Michael S. Bonkowski (374374)
Fernando P. Dominici (374376)
Oge Arum (374378)
Juliana S. Rocha (374380)
Khalid A. Al Regaiey (374381)
Reyhan Westbrook (374382)
Adam Spong (374384)
Jacob Panici (374386)
Michal M. Masternak (68106)
John J. Kopchick (331606)
Andrzej Bartke (68107)

February 2013

Liver protein isolates were immunoprecipitated (IP) with anti-pan-p85. Total p85α (A), 55α (B) an...

Representative Western blots for various proteins important in the control of muscle protein synthesis.

Jennifer L. Steiner (580144)
Anne M. Pruznak (580145)
Gina Deiter (580146)
Maithili Navaratnarajah (580147)
Lydia Kutzler (233164)
Scot R. Kimball (233175)
Charles H. Lang (318203)

June 2014

Immunoblots for total and/or phosphorylated AKT, 4E-BP1, 4E-BP2, and S6K1 are presented for both ...

Bar diagrams showing the levels of AKT (A), IRS-1 (B) and RPS6K (C) protein in adipose tissue, and PPARγ (D), mTOR (E), and RPS6K (F) protein in liver tissue, expressed as the mean ± sd of values from 8 animals aged 16 weeks, for control (CT) and BWF1 (SLE) mice.

Laia Vilà (295894)
Núria Roglans (295897)
Miguel Baena (295900)
Emma Barroso (295905)
Marta Alegret (295908)
Manuel Merlos (295911)
Juan C. Laguna (295915)

February 2013

The amount of protein loaded was confirmed by the Bradford method, and the uniformity of protein ...

Mean (+SEM) muscle and intra-abdominal adipose tissue mass.

Kerri Burgess (355665)
Tianshun Xu (355666)
Roger Brown (355667)
Bajin Han (355668)
Stephen Welle (272643)

February 2013

Myostatin-deficient (gray bars) and control mice (white bars) were fed a normal low-fat diet (13%...

GK and GKRP mRNA levels in livers from IRS-2(−/−), RIP-Irs-2/IRS-2(−/−) and their wild type mice.

Isabel Roncero (396876)
Elvira Alvarez (396877)
Carlos Acosta (396878)
Carmen Sanz (396879)
Pedro Barrio (396880)
Veronica Hurtado-Carneiro (396881)
Deborah Burks (396882)
Enrique Blázquez (396883)

April 2013

Levels of mRNA encoding GK (A) and GKRP (B) were measured in liver from IRS-2(−/−) ...

PGC-1α and exercise intensity dependent adaptations in mouse skeletal muscle - Fig 9

Nina Brandt (4524187)
Maja Munk Dethlefsen (4524184)
Jens Bangsbo (532981)
Henriette Pilegaard (215782)

October 2017

A) LC3II B) LC3I protein content, C) LC3II/LC3I Ratio D) and p62 protein content (AU) in quadrice...

iWAT HSLSer660 phosphorylation (a), eWAT HSLSer660 phosphorylation (b), iWAT P38Tyr180/182 phosphorylation (c), eWAT P38Tyr180/182 phosphorylation (d), iWAT GLUT4 protein content (e), iWAT GLUT4 protein content (f), iWAT (g) and eWAT in non-exercised mice as well as 6h or 10h after 2h of treadmill running in d IL-6 MKO and Control mice.

Jakob G. Knudsen (507454)
Anders Gudiksen (3165363)
Lærke Bertholdt (3165372)
Peter Overby (4691464)
Ida Villesen (4691461)
Camilla L. Schwartz (4691458)
Henriette Pilegaard (215782)

December 2017

(h) Representative blots of HSL565 phosphorylation, HSL, UCP1 and P38 protein content....

Relative expression of mRNAs encoding the lipogenic enzymes ME1 and FASN and pro-proliferative proteins mTOR and cyclin D1.

Ahmed Al-Dwairi (304346)
John Mark P. Pabona (304347)
Rosalia C. M. Simmen (304348)
Frank A. Simmen (304349)

February 2013

(A), Distal colons and (B), jejunums of WT and MOD-1 mice of the two diet groups. Data are mean ±...

Effects of Long-Term Caloric Restriction on Early Steps of the Insulin-Signaling System in Mouse Skeletal Muscle

Danila P. Argentino
O P. Dominici
Khalid Al-regaiey
Michael S. Bonkowski
Andrzej Bartke
Daniel Turyn

December 2014

In this study, we analyzed the effects of long-term (14 months) caloric restriction (CR) on the firs...

Skeletal muscle enzyme activity.

Christian Roy (303775)
Sabina Paglialunga (382945)
Gert Schaart (194711)
Esther Moonen-Kornips (382946)
Ruth C. Meex (382947)
Esther Phielix (284460)
Joris Hoeks (194696)
Matthijs K. C. Hesselink (357549)
Katherine Cianflone (155973)
Patrick Schrauwen (42320)

February 2013

HADH (A), CS (B) activities and HADH/CS ratio (C) for WT (white bars) and C5L2KO (black bars). Da...

Liver expression of LDLR and ABCA1 protein in mice with hepatic MTP knockdown.

Stephanie M. Marshall (223125)
Kathryn L. Kelley (505769)
Matthew A. Davis (505770)
Martha D. Wilson (350726)
Allison L. McDaniel (223120)
Richard G. Lee (505771)
Rosanne M. Crooke (361540)
Mark J. Graham (361539)
Lawrence L. Rudel (223108)
J. Mark Brown (505772)
Ryan E. Temel (223114)

January 2014

Following 6 weeks of control ASO or MTP ASO treatment, liver was collected for immunoblot analysi...

Muscle insulin signaling cascade total proteins and phospho-proteins in response to insulin stimulation (10 IU/kg) versus saline treated controls in GHRKO (KO) and normal (N) mice fed ad libitum (AL) or 30% calorie restriction (CR) for one year.

Michael S. Bonkowski (374374)
Fernando P. Dominici (374376)
Oge Arum (374378)
Juliana S. Rocha (374380)
Khalid A. Al Regaiey (374381)
Reyhan Westbrook (374382)
Adam Spong (374384)
Jacob Panici (374386)
Michal M. Masternak (68106)
John J. Kopchick (331606)
Andrzej Bartke (68107)

February 2013

Levels of total insulin receptor (IR; A), IR pY1158 (B), AKT1 (D), and AKT1 pS473 (E) were determ...

Metabolic parameters of male GHRKO (KO) and normal (N) mice fed ad libitum (AL) or subjected to 30% calorie restriction (CR) for one year.

Michael S. Bonkowski (374374)
Fernando P. Dominici (374376)
Oge Arum (374378)
Juliana S. Rocha (374380)
Khalid A. Al Regaiey (374381)
Reyhan Westbrook (374382)
Adam Spong (374384)
Jacob Panici (374386)
Michal M. Masternak (68106)
John J. Kopchick (331606)
Andrzej Bartke (68107)

February 2013

Panel (A) shows time-course changes in body weight over a one year period. After one year of CR, ...

Increased inflammation in skeletal muscle of KI mice is associated with reduced basal IRS1-PKB signalling.

Paul J. Meakin (800118)
Vicky L. Morrison (800119)
Claire C. Sneddon (800120)
Terhi Savinko (800121)
Liisa Uotila (800122)
Susan M. Jalicy (800123)
Jennie L. Gabriel (800124)
Li Kang (494757)
Michael L. J. Ashford (800125)
Susanna C. Fagerholm (800126)

September 2015

(A-C) Representative immunoblots for p-IRS1, IRS1, p-PKB and PKB, with quantification of immunobl...

Effect of procyanidins, EC and C3G on phosphorylation of IRS-1 in skeletal muscle of mice.

Yoko Yamashita (1948978)
Liuqing Wang (3106479)
Fumio Nanba (1948987)
Chiaki Ito (1948984)
Toshiya Toda (3106476)
Hitoshi Ashida (1948975)

September 2016

ICR mice were treated as described in <a href="http://www.plosone.org/article/info:doi/10.1371/jo...

Liver PI3K subunit abundance in GHRKO (KO) and normal (N) mice fed ad libitum (AL) or 30% calorie restriction (CR) for one year.

Michael S. Bonkowski (374374)
Fernando P. Dominici (374376)
Oge Arum (374378)
Juliana S. Rocha (374380)
Khalid A. Al Regaiey (374381)
Reyhan Westbrook (374382)
Adam Spong (374384)
Jacob Panici (374386)
Michal M. Masternak (68106)
John J. Kopchick (331606)
Andrzej Bartke (68107)

February 2013

Liver protein isolates were immunoprecipitated (IP) with anti-pan-p85. Total p85α (A), 55α (B) an...

Representative Western blots for various proteins important in the control of muscle protein synthesis.

Jennifer L. Steiner (580144)
Anne M. Pruznak (580145)
Gina Deiter (580146)
Maithili Navaratnarajah (580147)
Lydia Kutzler (233164)
Scot R. Kimball (233175)
Charles H. Lang (318203)

June 2014

Immunoblots for total and/or phosphorylated AKT, 4E-BP1, 4E-BP2, and S6K1 are presented for both ...

Bar diagrams showing the levels of AKT (A), IRS-1 (B) and RPS6K (C) protein in adipose tissue, and PPARγ (D), mTOR (E), and RPS6K (F) protein in liver tissue, expressed as the mean ± sd of values from 8 animals aged 16 weeks, for control (CT) and BWF1 (SLE) mice.

Laia Vilà (295894)
Núria Roglans (295897)
Miguel Baena (295900)
Emma Barroso (295905)
Marta Alegret (295908)
Manuel Merlos (295911)
Juan C. Laguna (295915)

February 2013

The amount of protein loaded was confirmed by the Bradford method, and the uniformity of protein ...

Mean (+SEM) muscle and intra-abdominal adipose tissue mass.

Kerri Burgess (355665)
Tianshun Xu (355666)
Roger Brown (355667)
Bajin Han (355668)
Stephen Welle (272643)

February 2013

Myostatin-deficient (gray bars) and control mice (white bars) were fed a normal low-fat diet (13%...

GK and GKRP mRNA levels in livers from IRS-2(−/−), RIP-Irs-2/IRS-2(−/−) and their wild type mice.

Isabel Roncero (396876)
Elvira Alvarez (396877)
Carlos Acosta (396878)
Carmen Sanz (396879)
Pedro Barrio (396880)
Veronica Hurtado-Carneiro (396881)
Deborah Burks (396882)
Enrique Blázquez (396883)

April 2013

Levels of mRNA encoding GK (A) and GKRP (B) were measured in liver from IRS-2(−/−) ...

PGC-1α and exercise intensity dependent adaptations in mouse skeletal muscle - Fig 9

Nina Brandt (4524187)
Maja Munk Dethlefsen (4524184)
Jens Bangsbo (532981)
Henriette Pilegaard (215782)

October 2017

A) LC3II B) LC3I protein content, C) LC3II/LC3I Ratio D) and p62 protein content (AU) in quadrice...

iWAT HSLSer660 phosphorylation (a), eWAT HSLSer660 phosphorylation (b), iWAT P38Tyr180/182 phosphorylation (c), eWAT P38Tyr180/182 phosphorylation (d), iWAT GLUT4 protein content (e), iWAT GLUT4 protein content (f), iWAT (g) and eWAT in non-exercised mice as well as 6h or 10h after 2h of treadmill running in d IL-6 MKO and Control mice.

Jakob G. Knudsen (507454)
Anders Gudiksen (3165363)
Lærke Bertholdt (3165372)
Peter Overby (4691464)
Ida Villesen (4691461)
Camilla L. Schwartz (4691458)
Henriette Pilegaard (215782)

December 2017

(h) Representative blots of HSL565 phosphorylation, HSL, UCP1 and P38 protein content....

Relative expression of mRNAs encoding the lipogenic enzymes ME1 and FASN and pro-proliferative proteins mTOR and cyclin D1.

Ahmed Al-Dwairi (304346)
John Mark P. Pabona (304347)
Rosalia C. M. Simmen (304348)
Frank A. Simmen (304349)

February 2013

(A), Distal colons and (B), jejunums of WT and MOD-1 mice of the two diet groups. Data are mean ±...

Effects of Long-Term Caloric Restriction on Early Steps of the Insulin-Signaling System in Mouse Skeletal Muscle

Danila P. Argentino
O P. Dominici
Khalid Al-regaiey
Michael S. Bonkowski
Andrzej Bartke
Daniel Turyn

December 2014

In this study, we analyzed the effects of long-term (14 months) caloric restriction (CR) on the firs...

Skeletal muscle enzyme activity.

Christian Roy (303775)
Sabina Paglialunga (382945)
Gert Schaart (194711)
Esther Moonen-Kornips (382946)
Ruth C. Meex (382947)
Esther Phielix (284460)
Joris Hoeks (194696)
Matthijs K. C. Hesselink (357549)
Katherine Cianflone (155973)
Patrick Schrauwen (42320)

February 2013

HADH (A), CS (B) activities and HADH/CS ratio (C) for WT (white bars) and C5L2KO (black bars). Da...

Liver expression of LDLR and ABCA1 protein in mice with hepatic MTP knockdown.

Stephanie M. Marshall (223125)
Kathryn L. Kelley (505769)
Matthew A. Davis (505770)
Martha D. Wilson (350726)
Allison L. McDaniel (223120)
Richard G. Lee (505771)
Rosanne M. Crooke (361540)
Mark J. Graham (361539)
Lawrence L. Rudel (223108)
J. Mark Brown (505772)
Ryan E. Temel (223114)

January 2014

Following 6 weeks of control ASO or MTP ASO treatment, liver was collected for immunoblot analysi...

Muscle total IRS-1 protein (A) and IRS-1 pS307 inhibitory phosphorylation (B) in GHRKO (KO) and normal (N) mice fed <i>ad libitum</i> (AL) or 30% calorie restriction (CR) for one year was determined using ELISA.

Abstract

Extracted data

Muscle total IRS-1 protein (A) and IRS-1 pS307 inhibitory phosphorylation (B) in GHRKO (KO) and normal (N) mice fed <i>ad libitum</i> (AL) or 30% calorie restriction (CR) for one year was determined using ELISA.

Abstract

Extracted data

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