Analysis of methylation and acetylation status of the RTA promoter <i>in vivo</i>.

<p>(A) RT-PCR detection of ORF52 and ORF73 expression. Infected tissues were prepared mice at 5, 16, 21 and 28 dpi and mRNA extracted. The coding sequences for ORF52 and ORF73 were RT-PCR amplified and separated on a 2% agarose gel. (B) COBRA of methylation status of RTA promoter. DNAs were extracted from infected tissue at 5, 8, 16, 21 or 28 dpi respectively, bisulfite treated, amplified by PCR, digested with Taqα| and separated on a 2% agarose gel. (C) TSA treatment of MHV-68 latently infected mice. At 28 dpi, MHV-68 infected BALB/C mice were administrated with TSA by subcutaneous injection in the groin (2.4 mg/kg). Five hrs post-induction, ChIP assay were used to detect histones H3 and H4 acetylation level at the RTA promoter.