shRNA-expressing plasmids mediate the allele-specific silencing of endogenous wild-type ataxin-3 in transiently transfected human 293T cells.

<p>A) Western blot of human 293T cells transfected with different shRNA (shAtaxWT, shAtaxMUT and shGFP)-expressing plasmids (5 µg; 48 h post-transfection). The blot clearly indicates that shAtaxWT downregulates endogenous/wild-type human ATX3, whereas shAtaxMUT has no silencing effect. shGFP was used as a control vector and the protein tubulin was used as a loading control. This western blot is representative of three independent experiments.