Comparison of BSX and R-BSX structures using CD and SDS-PAGE.

<p>(A) Far UV spectra of BSX and R-BSX. (B) CD spectra of thermal denaturation of BSX and R-BSX monitored at 222 nm with a temperature slope of 1°C/min. Note the difference in the thermal unfolding pattern between 40°C–60°C, marked by arrows. (C) SDS-PAGE analysis of BSX and R-BSX, showing higher mobility of R-BSX. To preserve their native conformations, samples were loaded onto the gel without prior boiling. (D) SwissPdb-generated structural models showing the N-terminal region of BSX and R-BSX. Additional hydrogen bonds between Met (start codon) and Gln2 are highlighted by colored dotted lines.