Intramolecular binding in BNIP-H.

<p>293T cells were transfected with expression plasmids for HA-BNIP-H full length, HA-BNIP-H aa 1–287 or HA-BNIP-H aa 1–190 alone or together with an expression construct for a FLAG-tagged C-terminal BNIP-H fragment (aa 286–371). A schematic picture of these fragments is shown (A). After co-immunoprecipitation with anti-FLAG antibody samples were analyzed by western-blotting with the indicated antibodies to reveal binding of the different HA-tagged fragments (B).