Pin1 competes with glutaminase for binding to BNIP-H in PC12 cells.

<p>PC12 cells were transfected with an expression plasmid for FLAG-BNIP-H full length alone or together with constructs for HA-Pin1 or HA-Rac1 as indicated. Cells were treated with NGF for 24 hours. After immunoprecipitation with anti-FLAG antibody, samples were analyzed by western-blotting with the indicated antibodies to reveal binding of endogenous kidney-type glutaminase to BNIP-H in the absence (lane 1) or presence of Pin1 (lane 2) or Rac1(lane 3). Samples shown were from the same experiment and analyzed on a single blot.