Generation of TANK1-deficient mice by gene targeting.

<p>(A) Gene targeting strategy and restriction map of TANK1 gene. Filled boxes indicate exons; labeled boxes indicate neomycin (neo) resistance or herpesvirus thymidine kinase (tk) genes; and arrows indicate loxP sites. (B, C) Southern blot analysis of ES cell DNA. The 4.2 kb and 1.5 kb bands represents the germ line alleles and 6.0 kb and 2.5 kb bands represent the targeted alleles when BamHI/XbaI enzymes were used (B). The 12 kb and 1.3 kb bands represent the germ line alleles, and 13.8 kb and 0.8 kb bands represent the targeted alleles when EcoRV was used(c). (D) PCR analysis for the conditional TANK1 knockout mouse genotype. The 340- and 240-bp PCR products represent the wild-type and floxed alleles, respectively. (E) PCR analysis for the constitutive TANK1 knockout mouse genotype. The 340- and 200-bp PCR products represented the wild-type and knockout alleles, respectively.