Generation of TG mice overexpressing βGalT1.

<p>(A) ES-cell targeting strategy, adapted from <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0079883#pone.0079883-Nyabi1" target="_blank">[25]</a>. Restriction enzyme sites and the location of the probe used for Southern blot analysis are depicted. The ROSA26 locus was targeted using the targeting vector (top) by homologous recombination. After antibiotics selection and Southern blot analysis, the ROSA26 locus was modified as shown in the figure. (B) Southern blot analysis on DNA derived from WT and heterozygous recombinant ES cells after <i>BamH</i>1 digestion and using the probe depicted in panel A. (C) PCR analysis on tail-extracted genomic DNA of two TG mice (with floxed βGalT1 alleles and containing cre) and two CT mice (mice with floxed βGalT alleles but not containing cre). PCR on genomic DNA was performed to detect the floxed Neomycin resistance gene (upper panel) and the cre gene (lower panel).