High-Dose Simvastatin Reduces Stress Fiber Formation and Alters RMEC Polarisation during Migration.

<p>(A) Immunocytochemistry for F-actin in green using the FITC-phalloidin probe. Scale bars 100 µm. (B) Labelling of the Golgi apparatus on the leading edge of migrating RMECs by staning for Golgin 97 in green. Nuclei are stained in red with PI. Scale bars 100 µm. (C) Identification of the MTOC on the leading edge of migrating RMECs by staning for α-tubulin in green. Nuclei are stained in red with PI. Scale bars 100 µm. (D) Accumulation of caveolin-1 on the trailing edge of migrating RMECs by immunocytochemistry in green. Nuclei are stained in red with PI. Scale bars 100 µm.