Effect of mitochondrial nutriments on expression of PPARGC1A, Nrf1 and Tfam.

<p>A: Western blot analysis in adipocytes of PPARGC1A. Quantitative values were tabulated with the ratio of densities of PPARGC1A: tubulin. Values are mean±SE of 12 animals in each group.*p<0.05, p<0.01 vs. Wistar control; # p<0.05, ##p<0.01 vs. GK control. B: Total RNA was isolated from soleus muscle. mRNA of <i>Nrf1</i> and <i>Tfam</i> were analyzed by means of quantitative RT-PCR with gene-specific oligonucleotide probes in muscle. The cycle number at which the various transcripts were detectable was compared to that of nuclear18S rRNA as an internal control, and expressed as arbitrary units versus values in Wistar control taken 100. All values are mean±SEM of 12 animals in each group.*p<0.05, ** p<0.01 vs. Wistar control; #p<0.05, ##p<0.01 vs. GK control.