Restoration of wild-type cell phenotype by expression of lamin A in <i>Lmna^−/−</i> MEFs.

<p><i>Lmna</i>^−/− MEFs were transfected with plasmids encoding either GFP (a–e) or GFP-lamin A (f–j). At 48 hours following transfection, cells were infected with HSV-1 at a MOI of 20 and processed for indirect immunofluorescence at 8 hpi. Antibodies for trimethyl H3K9 (b,g; blue) and ICP8 (c,h; red) were used. Double merged images of trimethyl H3K9 and ICP8 are shown in panels d and i. Triple merged images of GFP/GFP-lamin A (green), trimethyl H3K9 and ICP8 are shown in panels e and j. Scale bar = 5 µm.