RNA analysis by the Bioanalyzer 2100.

<p>(<i>A</i>) RNA isolated from fixed tissue by the optimized proteinase K/acid phenol method described here. (<i>B</i>) RNA isolated from frozen tissue by the optimized proteinase K/acid phenol method. (<i>C</i>) RNA isolated from fixed tissue by TRIzol method. (<i>D</i>) RNA isolated from fixed tissue by RNeasy Micro Kit. <i>(E)</i> One round of amplification of RNA isolated from fixed tissue by the optimized proteinase K/acid phenol method. (<i>F</i>) One round of amplification of Ambion Control RNA. (<i>G</i>) Two rounds of amplification of RNA isolated from fixed tissue by the optimized proteinase K/acid phenol method. (<i>H</i>) RNA ladder: first peak is RNA marker, next mark 200, 500, 1000, 2000, 4000 and 6000 nt.