Isolation of RNA from FF and FFPE tissues and generation of RNA-Seq libraries.

<p>(A) Bioanalyzer profiles and yields of RNA isolated from FF tissue and from sections of the matching FFPE block of a normal tonsil (reactive) sample. RNA was isolated in triplicate (representative profile shown) from sections of the FFPE block using three different purification kits. Yields of RNA and purification kits are stated in the text above each profile. (B) Bioanalyzer profiles of RNA (left) isolated by ExpressArt FFPE RNAready (Amp Tec) from FFPE samples of normal tonsil (reactive) stored for different times as indicated above the profile together with the yields. Isolations were conducted in triplicate and a representative profile is shown. The resulting profiles of RNA-Seq libraries (right, with library ID top-right), with or without preceding fragmentation of the RNA as indicated in the middle section.