Small RNA Sequencing and the Distribution of Small RNA Loci in Feature Classes

<p>(A) Flowchart for high-throughput sequencing and analysis of small RNAs. (B–G) Distribution of small RNAs from wt Col-0 plants and <i>rdr6–15, rdr1–1, rdr2–1, dcl1–7, dcl2–1, dcl3–1,</i> and <i>dcl4–2</i> mutants in the genome (B), transposons and retroelements (C), genes (D), pseudogenes (E), miRNAs (F), and tasiRNAs (G). In (B), the percentages of small RNAs in each of four size classes within each library are presented. In all other panels, normalized small RNA levels in each feature class are presented.