Hsp104 Inhibition Renders Sup35-GFP^{[<i>PSI+</i>]} Complexes Immobile

<div><p>(A) FRAP time courses for Sup35-GFP^{[<i>PSI+</i>]} in wild-type (SY360 × SY581; black circles) and in <i>+</i>/<i>KTKT</i> (SY893 × SY581; white squares) zygotes are shown (<i>n</i> = 6). The recovery half-time for the wild-type zygote is 0.92 s (R² = 0.85).</p> <p>(B) FRAP time courses for Sup35-GFP^{[<i>psi−</i>]} in wild-type (SY360 × SY582; black circles, <i>n</i> = 5) and in <i>+</i>/<i>KTKT</i> (SY893 × SY582; white squares, <i>n</i> = 6) zygotes are shown. The recovery half-time for the wild-type zygote is 0.47 s (R² = 0.85) and for the <i>+/KTKT</i> zygote is 0.7 s (R² = 0.89). The black arrow marks the bleach point. Error bars represent standard error of the mean.</p>