Sup35-GFP^{[<i>PSI+</i>]} Complexes, Which Persist after Hsp104 Inhibition, Remain Substrates for This Disaggregase upon Its Reactivation

<p>Wild-type zygotes (SY876 × 74D-694) constitutively expressing <i>SUP35</i> and <i>GST(UGA)DsRED</i> with existing Sup35-GFP (previously expressed from P<i>_MFA1</i>) were isolated in the presence of GdnHCl and allowed to form microcolonies. As indicated in the schematic diagram, a portion of this colony was retained on medium containing GdnHCl (0 h, left), and the remainder of the colony was transferred to medium lacking GdnHCl (0 h, right). The cells were imaged again 4 h after the transfer. Representative DIC, GFP, and DsRed images are shown (<i>n</i> = 9).