BSF-TPN Mutants Are Defective in Cell Separation

<p>(A–H) Phase-contrast images of BSF-TPN-B cells that were grown in the absence (−Tet) (A) or presence (+Tet) (B–H) of tetracycline for the indicated times. (B) and (C) are examples of cell clusters, where four cells are joined together at their extreme posterior tips. (D) is an example of three parallel cells that have failed to undergo cytokinesis. (E) is an example of a very large cell cluster, while (F–H) are examples of amorphous cells that ultimately predominate. The images shown in (G and H) are different focal planes of the same monstrous cell, which is a continuous mass on one face (G) and is clearly multi-flagellated on the other face (H). Scalebar is 10 μm.