Isolated mutants showing defects in the organization of microtubules or of the nuclear envelope.

<p>GFP-Atb2 (green), Nup40-mCherry (red), and Sfi1-CFP (blue) of isolated mutants are shown. All mutants were observed after increasing the temperature to 36°C for 3–6 h. Cells displaying the typical phenotype are marked with white arrowheads. The numbers of mutants are also shown. Mutants were categorized according to their phenotype (A–L), and a brief explanation of each phenotype is as follows. (<b>A</b>) Monopolar spindle. (<b>B</b>) The middle region of the spindle showed a dim GFP signal. (<b>C</b>) Accumulation of cells within the metaphase spindle. (<b>D</b>) The spindle was bent in anaphase. (<b>E</b>) Extremely short microtubules. (<b>F</b>) The number of microtubule bundles was fewer than in wild-type cells. (<b>G</b>) Microtubules were elongated and curved at cell tips. (<b>H</b>) Microtubules formed in the nucleus during interphase. (<b>I</b>) Microtubules were tethered around the cell tip. (<b>J</b>) The nuclear envelope was fragmented. (<b>K</b>) Cells had more than one nucleus. (<b>L</b>) Multi-septated cells. A differential interference contrast image of the outlined cell is also shown. Red arrowheads indicate septa. Scale bar: 5 µm.