Genetic and Molecular Analysis of the <i>ago2^dop</i> Mutations

<div><p>(A) Genetic characterization of the <i>dop</i> genomic region. Deletion mapping identified the cytological interval 71D1-E1 as the region uncovering the <i>dop</i> locus. Black bars represent the deleted regions. <i>Df(3L)BK10</i> represents the deletion that was used in the initial screen for female-sterile mutations by Galewsky and Schulz [<a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.0020134#pgen-0020134-b025" target="_blank">25</a>]. The distal breakpoint of <i>Df(3L)XG9</i> is to the right of the <i>mex1</i> gene (based on PCR mapping) and the proximal breakpoint is to the left of CG7739 (based on complementation of <i>l(3)s1754</i> and PCR probing for <i>CG7739</i>).</p><p>(B) Genomic organization of the 71D1 to 71E3 region as predicted from the release 3 of the annotated genomic sequence by the Berkeley <i>Drosophila</i> Genome Project. The 45-kb region contains six predicted genes and three P-element-insertions. The P-insertions <i>P(l(3)s1754)</i>, <i>P(l(3)03576,</i> and <i>P{EP}EP3417</i> were utilized to produce small deletions by male recombination (unpublished data).</p>