Functional Responses of Murine CD8⁺ T Cells Directed against Non-ts Epitopes

<div><p>(A) SCs from naive (gray bars) or chronically infected (Brazil strain; black bars) B6 mice were stimulated for 5 h with TSKB20, cruzipain, or GFT peptides plus GolgiPlug as described in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.0020077#s4" target="_blank">Materials and Methods</a>. Data represent the number of CD8⁺ T cells producing IFNγ; error bars represent SD.</p><p>(B) SCs from naive B6 mice were loaded with T. cruzi peptides and labeled with CFSE as described in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.0020077#s4" target="_blank">Materials and Methods</a>. The numbers above the peaks represent the percentage of specific killing of the target cells loaded with the indicated peptide and was calculated as described in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.0020077#s4" target="_blank">Materials and Methods</a>. Data are representative of three experiments.</p>