Functional profile of TcVac3-induced T cells in response to challenge infection by <i>T. cruzi</i>.

<p>Mice were injected with empty vector or TcVac3 (± cytokine adjuvants), infected with <i>T. cruzi,</i> and harvested at day 30 post-infection. (<b>A</b>) Splenic T cell profile was determined by flow cytometry after staining with FITC-conjugated anti-CD8 and PE-conjugated anti-CD4 antibodies. (<b>B–I</b>) Splenocytes were <i>in vitro</i> stimulated with recombinant antigens or TcTL for 48 h, and incubated with fluorescent-conjugated antibodies as described in Fig. 2. Shown are the CD4⁺ (<b>B–E</b>) and CD8⁺ (<b>F–I</b>) proliferating (Ki67⁺, B,C,F,G), and non-proliferating (Ki67⁻, D,E,H,I) T cell subsets and their intracellular cytokine (IFN-γ: C,D,G,H; IL-4: E&I<i>)</i> profile, measured by flow cytometry.